Research Article

A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies

Figure 5

The intercellular dendrite connection strength upon mechanical fluid movement for the nonattached dendrites in aLC explant cultures. The traces (b) represent the time courses of the 360/380 ratio , proportional to and correspond to the regions of interest (ROI) shown in the B&W image(a) in the same colors. (c) A series of the 360/380 ratio images at the time points are indicated. The values for are color coded with blue/green representing low ratio values and yellow/red representing high ratios (explant growth time: 21 days).
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(a)
232659.fig.005b
(b)
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(c)