The Impact of “Omic” and Imaging Technologies on Assessing the Host Immune Response to Biodefence Agents

Figure 1

ImageStreamX Mk1 imaging depicting intracellular infection of ex vivo and in vitro cells. (a) Infected and (b) uninfected ex vivo lung cells stained with fluorescently labelled antibodies specific for Burkholderia pseudomallei (Bps) and the neutrophil marker Ly6G. A composite (overlayed) fluorescence image shows intracellular Bps inside neutrophils (Ly6G/Bps). Mouse macrophage cell line (P388D.1) infected (c) in vitro with F. tularensis SCHU S4 (Ft) or uninfected controls (d). Each Image series shows 6 representative cells from one sample. SSC = Side Scatter; BF = Brightfield; FITC = Fluorescein Isothiocyanate; BV = Brilliant Violet.