Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy

Figure 1

Glomerular pathological changes in mice with diabetic nephropathy (DN) and control mice. (a) Renal morphology and glycogen accumulation at 25 weeks evaluated by hematoxylin and eosin (HE) and periodic acid Schiff (PAS) staining, renal interstitial fibrosis detected by Masson trichrome staining. (b) Relative area of mesangium matrix and basement membrane at different times. (c) Relative area of renal interstitial fibrosis at different times. In both experiments, more than 12 glomeruli were evaluated in each mouse. Magnification in (a) HE, PAS, Masson: 400x. ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus age-matched control mice. ^Δ𝑃<0.05, ^ΔΔ𝑃<0.01 versus 16-week-old DN mice. Scale bars, 50 μm. 𝑛=5 mice per group.

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