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Journal of Immunology Research
Volume 2014, Article ID 276345, 7 pages
http://dx.doi.org/10.1155/2014/276345
Research Article

Polymorphisms of Tumor Necrosis Factor-α, Transforming Growth Factor-β, and Interleukin-10 in Asthma Associated with Olive Pollen Sensitization

1Immunology Department, IIS-Fundación Jiménez Díaz, UAM, Avenida Reyes Católicos No. 2, 28040 Madrid, Spain
2CIBER de Enfermedades Respiratorias, CIBERES, Instituto de Salud Carlos III, Monforte de Lemos No. 3-5, 28029 Madrid, Spain
3Allergy Department, Hospital Universitario Vírgen del Rocío, Avenida Manuel Siurot, s/n, 41013 Seville, Spain

Received 27 October 2014; Revised 2 December 2014; Accepted 2 December 2014; Published 21 December 2014

Academic Editor: Kurt Blaser

Copyright © 2014 Blanca Cárdaba et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sensitization to specific olive pollen-allergens (Ole e 2 and 10) has been correlated with a clinical pattern of asthma. This study analyzes the association between several polymorphims of TNFA (G-308A, C-857T, and C-1031T), IL10 (C-571A and A-1117G), and TGFB (C-509-T) and these sensitizations. These polymorphisms were genotyped by allelic discrimination, in olive pollen-allergic patients (phenotyped for specific Ole e 2 and 10 sensitizations) and healthy controls. Levels of serum-soluble cytokines were correlated with specific genotypes and clinical phenotypes. The results showed that heterozygous TGFB C-509T genotype, besides having the lowest sera TGF- levels, was significantly increased in olive pollen-allergic patients compared with controls. According specific sensitizations, CC genotype of IL10 C-571A could be a protective factor for Ole e 2 sensitization and mainly for asthmatic Ole e 2 sensitized patients compared with asthmatic non-Ole e 2 sensitized patients (OR: 0.26, ). In contrast, heterozygous CA genotype was increased in Ole e 2 asthmatic subjects compared to asthmatic non-Ole e 2 sensitized patients. Lastly, heterozygous TNFA G-308A genotype was associated with Ole e 10 sensitization (OR: 2.5, ). In conclusion, these results suggest a role of TGF-β1 in olive-pollen sensitization and TNF-α and IL-10 genotypes in the asthma induced by specific olive-pollen allergens.