Anti-MDA5 Antibodies in a Large Mediterranean Population of Adults with Dermatomyositis

Figure 1

Immunoblots showing the reactivity of IgG antibodies from dermatomyositis patients against commercially available purified recombinant MDA5. Lanes 3 and 4 (+), 5 and 6 (++), and 7 and 8 (+++) were considered positive results. Lanes 1 and 2 corresponded to negative serum samples. Dashed arrows are probably degradation products of MDA5.