IFN-γ-Secreting-Mesenchymal Stem Cells Exert an Antitumor Effect In Vivo via the TRAIL Pathway

Figure 3

MSCs IFN-γ selectively induced apoptosis in cancer cells, but not in normal cells. MSCs IFN-γ were cocultured with 3T3 hrGFP (a), 293FT hrGFP (b), H460 hrGFP (c), H1299 hrGFP (d), A549 hrGFP (e), and MCF-7 hrGFP (f) cells for 48 hours. The mixed cells were observed using phase-contrast, GFP-specific, and DAPI-specific microscopy. The typical apoptotic and dead cells are marked. Scale bar: 200 μm. (g) The cell viability within the coculture system was measured using a CCK-8 kit and was shown as a percentage normalized to the viability of the control MSCs coculture group, which was set to 100%. The asterisks indicate 𝑃<0.01. The reported results are representative of the results from three separate experiments.

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