Autoantibodies Targeting AT1 Receptor from Patients with Acute Coronary Syndrome Upregulate Proinflammatory Cytokines Expression in Endothelial Cells Involving NF-κB Pathway

Figure 3

IL-6, VCAM-1, and MCP-1 mRNA expression in human endothelial cells stimulated by AT1-AAs. In cells incubated with AT1-AAs (20 μg/mL) for 0, 1, 4, 8, 12, and 24 hours or human nonspecific IgG (20 μg/mL) from the patients for 8 hours as a negative control, the IL-6 (panel (a)), VCAM-1 (panel (b)), and MCP-1 (panel (c)) mRNA levels were detected by real-time quantitative PCR analysis. -axis represents the relative mRNA levels; ; versus 0 hour (control); . (nonspecific IgG group ).

(a)

(b)

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