Construction of a Chimeric Secretory IgA and Its Neutralization Activity against Avian Influenza Virus H5N1

Table 1

Primers used in this assay.

Primer name	Primer sequence
mVK-f-ATG	ATGGAGWCAGACACACTCCT
mVK-r	GGATACAGTTGGTGCAGCATC
mVH-f-ATG	ATGGRATGGAGCTGGATCTT
mVH-r	ATAGACAGATGGGGGTGTCGTTTTGGC
IGA-mVHhCHf	TCTCCTCAGCATCCCCGACCAGCCCCAA
IGA-mVHhCHr	TCGGGGATGCTGAGGAGACGGTGACTGA
IGA-Hf-EcoRI	CGGAATTCACCACCATGGGATGGAGCTGGATCT
IGA-Hr-Xbal	GCTCTAGATCAGTAGCAGGTGCCGTCCA
IGA-mVKhCKf	AATCAAACGAACTGTGGCTGCACCATCT
IGA-mVKhCKr	CCACAGTTCGTTTGATTTCCAGCTTGGT
IGA-Kf-EcoRI	CGGAATTCACCACCATGGAGACAGACACACTCCT
IGA-Kr-Xbal	GCTCTAGACTAACACTCTCCCCTGTTGAAGCTCTTTGTGA