A Mycobacterium bovis BCG-Naked DNA Prime-Boost Vaccination Strategy Induced CD4⁺ and CD8⁺ T-Cell Response against Mycobacterium tuberculosis Immunogens

Figure 2

Analysis of the lymphoproliferative response to the prime-boost approach. Splenocytes were prepared from individual mice after the animals were killed, and proliferation was analyzed by MTT method using the TB-PPD stimulating as described in Section 2. The proliferation was measured by SI, and the data are presented as means ± standard errors. ^∗𝑃<0.01 versus PBST, BCG-C, BCG-P, BCG-C+pcDNA3.1, or BCG-P+pcDNA3.1 groups. ^#𝑃<0.05 versus BCG-C+3.1-72 group. ^◊𝑃<0.05 versus BCG-C+3.1-72 or BCG-P+3.1-72 group. ^☆𝑃<0.05 versus BCG-P+3.1-69, BCG-C+3.1-72, or BCG-P+3.1-72 groups.