Multiplex Evaluation of Influenza Neutralizing Antibodies with Potential Applicability to In-Field Serological Studies
The influence of different storage temperatures on pseudotype virus titre. The stability of H5N1 lacZ pseudotypes was evaluated by storing aliquots of virus at different temperatures for up to 6 months. Pseudotype titres for the time course are given relative to the titre of virus stocks maintained at −80°C which is considered the optimal storage temperature for retroviral pseudotypes. One biological replicate pseudotype virus was used to generate three technical replicates. Error bars represent standard deviation.