Disruption of the Suprachiasmatic Nucleus Blunts a Time of Day-Dependent Variation in Systemic Anaphylactic Reaction in Mice
Figure 2
The SCN ablation blunts a time of day-dependent variation in PSA reaction. (a) Changes in rectal temperature over time after the allergen challenge at ZT4 (10:00 a.m.) and ZT16 (10:00 p.m.) in sham-operated mice (Sham) and mice with disrupted SCN (SCND) (). The arrows indicate the time of antigen challenge (b)–(d). The serum histamine (b), MCP-1 (CCL2) (c), and IL-6 (d) levels following induction of PSA reactions at ZT4 and ZT16 in sham-operated mice or mice with SCN disruption ( per group). Concentrations of histamine were measured using serum samples collected at 10 and 180 minutes after induction of PSA reaction at ZT4 or ZT10. Concentrations of MCP-1 and IL-6 were measured using serum samples collected at 180 minutes after induction of PSA reaction at ZT4 or ZT16. (e) The serum corticosterone levels at ZT4 and ZT16 in sham-operated mice and mice with SCN disruption ( per group). . Similar results were obtained in at least two independent experiments.
