Parainfluenza Virus Type 1 Induces Epithelial IL-8 Production via p38-MAPK Signalling
Figure 6
JNK phosphorylation. Detection of JNK phosphorylation in HEp-2 and A549 cells infected with HPIV-1. Cells were infected with live and inactive HPIV-1 at different time points, and JNK phosphorylation was detected. Whole cell lysates were prepared for Western blotting (as described in Materials and Methods) to detect the phosphorylated (active) forms of the kinases: (a) phospho-JNK was detected with monoclonal antibody sc-135642 and the total JNK sc-571 in HEp-2 cells; (b) densitometric analysis of JNK protein concentrations; (c) phospho-JNK and total protein concentrations in A549 cells; (d) densitometric analysis of the protein concentrations. Mp: molecular weight; CTL: control and UV (inactive virus) that corresponds to 12 hours in path 3. Data are the means for triplicate measurements from the three separate experiments.
