Alpha/Beta T-Cell Depleted Grafts as an Immunological Booster to Treat Graft Failure after Hematopoietic Stem Cell Transplantation with HLA-Matched Related and Unrelated Donors

Table 3

Multiplex PCR reactions used for spectratyping.

Multiplex	Primer
TCR-1	VfI + V10 + J1.3/2.3 (NED*) + J1.1/2.1 (FAM*)
TCR-2	V11 + J1.3/2.3 (NED) + J1.1/2.1 (FAM)
TCR-3	V9 + J1.3/2.3 (NED) + J1.1/2.1 (FAM)
TCR-4	V9 + J1.2^† (FAM)
TCR-1	V1 (FAM) + V2 (NED) + J1
TCR-2	V1 (FAM) + V2 (NED) + J2
TCR-3	V1 (FAM) + V2 (NED) + J3
TCR-4	V1 (FAM) + V2 (NED) + J4

Primers were labelled with either NED or 6-FAM.
Primer sequences can be found in [26].
^†J1.2 sequence: AAGAAAACTTACCTGTAATGATAAGC.