Review Article
Some Basic Aspects of HLA-G Biology
Table 1
Examples of methods developed for measuring soluble HLA-G.
| Type | Standard | Capture antibody | Detection antibody | Detection | Detection limit | Reference |
| ELISA-sandwich | HLA-G1/LCL 722.221 transfected cells | W6.32 after depletion with TP25.99 | anti-2-m | Colorimetric | 2.1 ng/mL | [55] |
| ELISA-sandwich | None | 87G, BFL.1 or MEM-G/9 | W6/32 | Colorimetric | O.D. | [56] |
| ELISA-sandwich | HLA-G transfected CHO cells | G233 | 56B | Colorimetric | 1 ng/mL | [57] |
| ELISA-sandwich | HLA-G5 protein derived from insect SF9 cells | MEM-G/9 | anti-2-m | Colorimetric | 5 ng/mL | [58] |
| ELISA-sandwich | HLA-G5 protein derived from insect SF9 cells | 5A6G7 | W6/32 | Colorimetric | 5 ng/mL | [58] |
| Luminex | HLA-G5 and beta2 m transfected SF9 cells | MEM-G9 | anti-2-m | Fluorescence | 0.3 ng/mL | [59] |
| ELISA-sandwich | HLA-G transfected LCL 721.221 cells | MEM-G/9 | W6/32 | Fluorescence | 1 ng/mL | [60] |
| Bio-Plex | HLA-G5 transfected HeLa cells | MEM-G/9 | W6/32 | Fluorescence | 0.3 ng/mL | [61] |
| ELISA-sandwich | HLA-G transfected LCL 721.221 cells | MEM-G/9 | W6/32 | Chemiluminescence | 2 ng/mL | [62] |
| ELISA-sandwich | Purified HLA-G | HGY (noncommercial) | Polyclonal anti-HLA-G | Colorimetric | 1 U/mL | [63] |
| ELISA-sandwich | HLA-G1 transfected LCL-721.221 cells | G233 | anti-2-m | Colorimetric | 4 ng/mL | [64] |
|
|