Some Basic Aspects of HLA-G Biology

Table 1

Examples of methods developed for measuring soluble HLA-G.

Type	Standard	Capture antibody	Detection antibody	Detection	Detection limit	Reference
ELISA-sandwich	HLA-G1/LCL 722.221 transfected cells	W6.32 after depletion with TP25.99	anti-𝛽2-m	Colorimetric	2.1 ng/mL	[55]
ELISA-sandwich	None	87G, BFL.1 or MEM-G/9	W6/32	Colorimetric	O.D.	[56]
ELISA-sandwich	HLA-G transfected CHO cells	G233	56B	Colorimetric	1 ng/mL	[57]
ELISA-sandwich	HLA-G5 protein derived from insect SF9 cells	MEM-G/9	anti-𝛽2-m	Colorimetric	5 ng/mL	[58]
ELISA-sandwich	HLA-G5 protein derived from insect SF9 cells	5A6G7	W6/32	Colorimetric	5 ng/mL	[58]
Luminex	HLA-G5 and beta2 m transfected SF9 cells	MEM-G9	anti-𝛽2-m	Fluorescence	0.3 ng/mL	[59]
ELISA-sandwich	HLA-G transfected LCL 721.221 cells	MEM-G/9	W6/32	Fluorescence	1 ng/mL	[60]
Bio-Plex	HLA-G5 transfected HeLa cells	MEM-G/9	W6/32	Fluorescence	0.3 ng/mL	[61]
ELISA-sandwich	HLA-G transfected LCL 721.221 cells	MEM-G/9	W6/32	Chemiluminescence	2 ng/mL	[62]
ELISA-sandwich	Purified HLA-G	HGY (noncommercial)	Polyclonal anti-HLA-G	Colorimetric	1 U/mL	[63]
ELISA-sandwich	HLA-G1 transfected LCL-721.221 cells	G233	anti-𝛽2-m	Colorimetric	4 ng/mL	[64]