Genetic Diversity of MSP1 Block 2 of Plasmodium vivax Isolates from Manaus (Central Brazilian Amazon)
Figure 2
Analysis of diversity of PCR products of PvMSP1 Block 2. (a) Agarose gel showing fragment size of PCR products of the PvMSP1 Block 2 in 17 samples. Based on 100 bp molecular weight ladder (MW), four different types of fragments ranging, and 500, 530, 550 between 600 base pairs were defined by calculating the ratio of the distance of known bands (right side). (b) Distributions of fragments per isolate and frequencies of each type of fragment are shown. (c) Based on amino acid sequences alignment of PvMSP1 Block 2, seven haplotypes could be classified by short tandem in positions 10° to 70°. At the top of the alignment is consensus sequence. (d) Prevalence of seven haplotypes among field isolates in Manaus.
