Genetic Diversity of MSP1 Block 2 of Plasmodium vivax Isolates from Manaus (Central Brazilian Amazon)
Figure 4
Occurrence of nucleotide diversity by non synonymous and synonymous mutations in polymorphic region of Block 2. (a) Using Blast, the sequence GQ890943 was similar to the haplotypes of isolate 10. Only nucleotide substitutions were shown in the panel with colonies of E. coli containing PCR products of Block 2 from isolate 10 cloned into plasmids. (b) The same was evaluated with PCR products of Block 2 from isolate 15. The sequence AF435623 was selected using BLAST and only nucleotide substitutions were shown in the panel. (c) The sequences encompassing Block 2, (interspecies conserved blocks (ICB) 1 and 2) were represented. Prediction of linear B-cell epitopes was carried out and underlined by bars [25]. Differential binding of T-cell epitopes was predicted for all HLA-DRB alleles accessible into the ProPred using MHC class II binding peptide prediction server [26]. We observed several T-cell epitopes (blue letters) with residue anchor (red letter). Location of nonsynonymous substitutions was represented by black dots. Superior sequence (GQ890943) was similar to haplotypes of isolate 10. Inferior sequence (AF435623) was similar to haplotypes of isolate 15.
