Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases

Figure 2

Surface staining of CD3, CD56, CD7, CD11c, CD20, CD22, and CD27 epitopes with methanol-based permeabilization. Freshly isolated human PBMCs from a healthy donor were fixed using 2% paraformaldehyde, permeabilized in methanol, and stained with MoAb clones against CD3, CD56, CD7, CD11c, CD22, and CD27. Lymphocytes were gated on forward/side light scatter properties. Successful discrimination of CD56+CD3−, CD56+CD3+, CD56−CD3+, CD56+CD11c+, CD56+CD11c−, CD56+CD7+, CD22+CD27+, and CD22+CD27− cells was demonstrated. A clear distinction between high CD56 and medium/low CD56 as well as high CD7 and medium/low CD7 was noted (first row, right plot and second row, left plot, resp.).