Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases
Figure 7
Simultaneous detection of IFN-γ, IL-10, and p-p38 MAPK expression in PBMCs cell subpopulations. (a) shows the plots for CD11c versus IFN-γ and IFN- versus p-p38. (b) shows the plots for CD3 versus IL-10 and IL-10 versus p-p38. In addition to IFN-, IL-10 can be detected under harsh methanol permeabilization conditions. IL-10 expression precedes that of IFN-γ and can be seen in both CD3+ and CD3− cells.
