Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo
Figure 2
Neutrophil number in the peritoneal cavity of mice treated with casein and warifteine. (a, b) Cell number evaluation by flow cytometry. Peritoneal cells were obtained from mice after treatment with warifteine and casein. The cells were prepared for analysis by flow cytometry. (a) Side scatter and forward scatter profiles of normal (left) and casein-treated (right) animals to indicate the R1 gate. (b) Total PMN cell number in the R1 gate (as defined in (a)). The FSC versus SSC plots are representative of three independent experiments. The mean value of neutrophil numbers in R1 gates from three independent experiments is shown ( animals per group/experiment). as compared to untreated controls and as compared to casein-treated animals. (c) Analysis of the percentage of Gr1+ cells in the peritoneal cavity fluid from animals treated with casein and warifteine. Peritoneal fluid was obtained from warifteine and casein-treated animals. The samples were incubated with an anti-Gr1-FITC labeled antibody. Histograms show the Gr1-expressing cells in the R1 gate. The value shown in M1 indicates the percentage of Gr1+ cells. Data shown in the histogram plots are representative of two independent experiments. (d) Quantification of the number of Gr1+ cells in the R1 gate. Data shown indicate the total number of GR1+ cells from experiments shown in (a) and are the mean value from two experiments ( animals per group/experiment): in comparison with control animals and in relation to animals treated with casein alone. (e) Percentage of neutrophils in peritoneal exudate obtained from mice treated with casein and warifteine. Peritoneal exudates were obtained after a treatment with warifteine and casein and were submitted to cytospin followed by May-Grunwald Giemsa staining. Data in (e) show the percentage of neutrophils in the samples obtained by differential cell count. Data shown are mean value from six independent experiments ( animals per group/experiment). when comparing with untreated controls and when comparing data with those from the group treated with casein only.
