Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo
Figure 4
Measurement of reactive oxygen species in cultures stimulated with PMA and treated with warifteine. Neutrophils were incubated in either the presence or absence of PMA. Some cultures were also treated with the indicated doses of warifteine. The samples were then incubated with the probe CM-H2DCFDA. The production of ROS was analyzed by flow cytometry. The M1 marker shows the percentage of cells that metabolized the probe. The data are representative of three independent experiments.
