Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo
Figure 6
Adhesion of neutrophils to CHO cells and to CHO cells expressing P and E selectin. CHO cells transfected with either P (CHOP) or E selectin (CHOE) genes were used. A cell monolayer was obtained by overnight incubation of normal or transfected CHO cells in coverslips. Peritoneal exudate neutrophils were obtained and treated with warifteine in vitro. Those cells were then transferred to the monolayers of CHO cells and incubated. Coverslips were removed, washed with PBS, and Giemsa-stained. They were analyzed by optical microscopy. The number of neutrophils adhered to CHO cells was scored. The graphs shown indicate the mean values from four independent experiments performed in triplicate. in the comparison of cultures treated with warifteine (in different concentrations) and cultures treated with medium only without warifteine.
