Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo
Figure 7
Inhibition of neutrophil migration in the presence of warifteine and induction of increased intracellular cAMP levels. (a) Solutions of chemoattractant (fMLP) were added to a 24-well plate. A neutrophil suspension was obtained and either left untreated or incubated with the indicated doses of warifteine. Theses samples were added to transwell inserts which were placed into the fMLP filled wells. Cell migration was determined by counting cell number in the outside (fMLP) compartment using a hemocytometer. The chemotactic index was calculated for each sample. Data shown indicate the mean value obtained from two independent experiments. when comparing untreated and fMLP-stimulated cultures; when comparing data from warifteine-treated and untreated cultures. (b) Samples obtained as described in (a) were incubated with warifteine, DbAMPc, or forskolin. Cultures were set up as in (a). Pretreated cell samples were added into a transwell insert placed in fMLP containing wells (outside compartment). The chemotactic index is shown. (c) Neutrophil cultures were incubated with either forskolin or warifteine. Control cultures received no pretreatment. Intracellular cAMP levels were measured by a competitive binding assay. Data indicate the fold increase of cAMP levels compared to untreated controls (set as 1).
