Warifteine, an Alkaloid Purified from Cissampelos sympodialis, Inhibits Neutrophil Migration In Vitro and In Vivo
Figure 8
Extracellular DNA traps (NETs) induction in warifteine-treated neutrophil cultures. (a) Neutrophils were preincubated with the indicated doses of warifteine. The cells were then stimulated with fMLP. The supernatants were then harvested and the amount of extracellular DNA was measured. (b) Neutrophils were transferred to coverslips, treated with warifteine (2 μM), and then stimulated with fMLP. The coverslips were fixed and stained with DAPI. This was followed by analysis by phase contrast and fluorescence microscopy. Plots show representative fields recorded in light (right) and dark (left) fields where DAPI labeling was determined. Data are representative of three independent experiments.
