Photobacteriosis: Prevention and Diagnosis

Table 2

Methods for direct identification of Photobacterium damselae subsp. piscicida.

Assay	Target	Additional culture step	Specificity	Availability on the market	References
PCR-based detection method	16S gene	—	P. damselae		Osorio et al. [42]
Multiplex PCR assay	16S gene; ureC gene	—	P. damselae subspecies		Osorio et al. [1]
Multiplex PCR assay	Pbp-1A gene; ureC gene; internal amplification control	—	P. damselae subspecies	Photobacterium damselae-PCR detection Kit by Diatheva	Amagliani et al. [43]
PCR technique and plating method	cps gene	TCBS-1 agar	P. damselae subspecies		Rajan et al. [44]
Enzyme immunoassay	Polyclonal antibodies against P. damselae subsp. piscicida	—	P. damselae subsp. piscicida a	Aquaeia-Pp kit by BIONOR	Romalde et al. [45]
PCR-RFLP method	GenBank AY191120,; AY191121 sequences	—	P. damselae,; restriction analysis for subspecies identification		Zappulli et al. [46]

aCross reactions with P. damselae subsp. damselae and P. histaminum.