Review Article
Photobacteriosis: Prevention and Diagnosis
Table 2
Methods for direct identification of Photobacterium damselae subsp. piscicida.
| Assay | Target | Additional culture step | Specificity | Availability on the market | References |
| PCR-based detection method | 16S gene | — | P. damselae | | Osorio et al. [42] |
| Multiplex PCR assay | 16S gene ureC gene | — | P. damselae subspecies | | Osorio et al. [1] |
| Multiplex PCR assay | Pbp-1A gene ureC gene internal amplification control | — | P. damselae subspecies | Photobacterium damselae-PCR detection Kit by Diatheva | Amagliani et al. [43] |
| PCR technique and plating method | cps gene | TCBS-1 agar | P. damselae subspecies | | Rajan et al. [44] |
| Enzyme immunoassay | Polyclonal antibodies against P. damselae subsp. piscicida | — | P. damselae subsp. piscicida | Aquaeia-Pp kit by BIONOR | Romalde et al. [45] |
| PCR-RFLP method | GenBank AY191120, AY191121 sequences | — | P. damselae, restriction analysis for subspecies identification | | Zappulli et al. [46] |
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Cross reactions with P. damselae subsp. damselae and P. histaminum.
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