Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR
Figure 2
Standard curves were generated based on Cp values of 10-fold dilutions of plasmid DNA. Regression lines between the Cp () values and the input concentrations of HP-PRRSV (a) and PRRSV (b) plasmid DNA in real-time RT-PCR detected using SYBR Green I and HP-PRRSV (c) and PRRSV (d) using TaqMan probe, respectively.