Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR
Figure 3
Specific amplification curves and melting curve analysis by SYBR Green I real-time PCR. (a) Specific amplification curves. Fluorescent curves were observed when HP-PRRSV (GD) and PRRSV (CH-1a) were used as templates; no fluorescent signals were observed when the templates were other viruses and host cells. (b) Melting curves. Tm of HP-°C; Tm of °C.
