Table of Contents Author Guidelines Submit a Manuscript
Journal of Immunology Research
Volume 2014, Article ID 809656, 7 pages
http://dx.doi.org/10.1155/2014/809656
Research Article

Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR

State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China

Received 24 November 2013; Accepted 15 May 2014; Published 12 June 2014

Academic Editor: Yong-Suk Jang

Copyright © 2014 Shuqi Xiao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. Xiao, J. Jia, D. Mo et al., “Understanding PRRSV infection in porcine lung based on genome-wide transcriptome response identified by deep sequencing,” PLoS ONE, vol. 5, no. 6, Article ID e11377, 2010. View at Publisher · View at Google Scholar · View at Scopus
  2. K. Tian, X. Yu, T. Zhao et al., “Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark,” PLoS ONE, vol. 2, no. 6, article e526, 2007. View at Publisher · View at Google Scholar · View at Scopus
  3. Y. Li, X. Wang, K. Bo et al., “Emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus in the Mid-Eastern region of China,” Veterinary Journal, vol. 174, no. 3, pp. 577–584, 2007. View at Publisher · View at Google Scholar · View at Scopus
  4. G.-Z. Tong, Y.-J. Zhou, X.-F. Hao, Z.-J. Tian, T.-Q. An, and H.-J. Qiu, “Highly pathogenic porcine reproductive and respiratory syndrome, China,” Emerging Infectious Diseases, vol. 13, no. 9, pp. 1434–1436, 2007. View at Google Scholar · View at Scopus
  5. Y. Feng, T. Zhao, T. Nguyen et al., “Porcine respiratory and reproductive syndrome virus variants, Vietnam and China, 2007,” Emerging Infectious Diseases, vol. 14, no. 11, pp. 1774–1776, 2008. View at Publisher · View at Google Scholar · View at Scopus
  6. M. Ying, Y. Feng, D. Liu, and G. F. Gao, “Avian influenza virus, streptococcus suis serotype 2, severe acute respiratory syndrome-coronavirus and beyond: molecular epidemiology, ecology and the situation in China,” Philosophical Transactions of the Royal Society B: Biological Sciences, vol. 364, no. 1530, pp. 2725–2737, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. D. Normile, “China, Vietnam grapple with “rapidly evolving” pig virus,” Science, vol. 317, no. 5841, p. 1017, 2007. View at Publisher · View at Google Scholar · View at Scopus
  8. Y.-J. Zhou, X.-F. Hao, Z.-J. Tian et al., “Highly virulent porcine reproductive and respiratory syndrome virus emerged in China,” Transboundary and Emerging Diseases, vol. 55, no. 3-4, pp. 152–164, 2008. View at Publisher · View at Google Scholar · View at Scopus
  9. W. Lurchachaiwong, S. Payungporn, U. Srisatidnarakul, C. Mungkundar, A. Theamboonlers, and Y. Poovorawan, “Rapid detection and strain identification of porcine reproductive and respiratory syndrome virus (PRRSV) by real-time RT-PCR,” Letters in Applied Microbiology, vol. 46, no. 1, pp. 55–60, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. E. Martínez, P. Riera, M. Sitjà, Y. Fang, S. Oliveira, and J. Maldonado, “Simultaneous detection and genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) by real-time RT-PCR and amplicon melting curve analysis using SYBR Green,” Research in Veterinary Science, vol. 85, no. 1, pp. 184–193, 2008. View at Publisher · View at Google Scholar · View at Scopus
  11. A. Wasilk, J. D. Callahan, J. Christopher-Hennings et al., “Detection of U.S., lelystad, and european-like porcine reproductive and respiratory syndrome viruses and relative quantitation in boar semen and serum samples by real-time PCR,” Journal of Clinical Microbiology, vol. 42, no. 10, pp. 4453–4461, 2004. View at Publisher · View at Google Scholar · View at Scopus
  12. X.-L. Xiao, H. Wu, Y.-G. Yu et al., “Rapid detection of a highly virulent Chinese-type isolate of Porcine Reproductive and Respiratory Syndrome virus by real-time reverse transcriptase PCR,” Journal of Virological Methods, vol. 149, no. 1, pp. 49–55, 2008. View at Publisher · View at Google Scholar · View at Scopus
  13. S. Xiao, Q. Wang, J. Jia et al., “Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by two-dimensional fluorescence difference gel electrophoresis,” Virology Journal, vol. 7, article 107, 2010. View at Publisher · View at Google Scholar · View at Scopus
  14. Z.-Z. Yang, W.-H. Fang, and M. Habib, “First results of detection of PRRSV and CSFV RNA by SYBR green I-based quantitative PCR,” Journal of Veterinary Medicine B: Infectious Diseases and Veterinary Public Health, vol. 53, no. 10, pp. 461–467, 2006. View at Publisher · View at Google Scholar · View at Scopus
  15. H. Tian, J. Wu, Y. Shang, Y. Chen, and X. Liu, “The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus,” Virology Journal, vol. 7, article 90, 2010. View at Publisher · View at Google Scholar · View at Scopus
  16. S. B. Kleiboeker, S. K. Schommer, S.-M. Lee, S. Watkins, W. Chittick, and D. Polson, “Simultaneous detection of North American and European porcine reproductive and respiratory syndrome virus using real-time quantitative reverse transcriptase-PCR,” Journal of Veterinary Diagnostic Investigation, vol. 17, no. 2, pp. 165–170, 2005. View at Google Scholar · View at Scopus