Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR

Table 1

Primers and probes used in the real-time RT-PCR and conventional RT-PCR assays.

Primers and probes		Sequences (5′-3′)	Products (bp)
			HP-PRRSV	PRRSV
Conventional RT-PCR	NSP2-F	AACACCCAGGCGACTTCA	787	874
	NSP2-R	GCATGTCAACCCTATCCCAC
Real-time RT-PCR	NSP2-qF	GTGGGTCGGCACCAGTT	85	172
	NSP2-qR	GACGCAGACAAATCCAGAGG
Probes	Pb-H	FAM-CGCGTAGAACTGTGACAACAACGCTGA-TAMRA [12]
	Pb-N	HEX-AAAATTGGCTCACTCAAGGGCGTCA-TAMRA
	Pb-all	FAM-CACAGTTCTACGCGGTGCAGG-TAMRA