Research Article
Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR
Table 2
Intra- and interassay reproducibility of real-time PCR.
| Concentration of standard plasmid (copies/L) | | Intra-assay (Cp) | Interassay (Cp) | Mean | SD | CV (%) | Mean | SD | CV (%) |
| HP-PRRSV (SYBR) | | | | | | | | | 3 | 14.96 | 0.02 | 0.13 | 14.82 | 0.29 | 1.96 | | 3 | 21.78 | 0.04 | 0.18 | 21.49 | 0.57 | 2.65 | | 3 | 28.5 | 0.16 | 0.56 | 28.48 | 0.27 | 0.95 | PRRSV (SYBR) | | | | | | | | | 3 | 15.47 | 0.02 | 0.13 | 15.75 | 0.31 | 1.97 | | 3 | 22.55 | 0.05 | 0.22 | 22.71 | 0.19 | 0.84 | | 3 | 29.6 | 0.01 | 0.03 | 29.96 | 0.34 | 1.13 | HP-PRRSV (FAM) | | | | | | | | | 3 | 15.9 | 0.02 | 0.13 | 15.98 | 0.06 | 0.38 | | 3 | 22.51 | 0.01 | 0.04 | 22.63 | 0.15 | 0.66 | | 3 | 29.71 | 0.02 | 0.07 | 29.59 | 0.17 | 0.57 | PRRSV (HEX) | | | | | | | | | 3 | 16.59 | 0.15 | 0.90 | 16.47 | 0.04 | 0.24 | | 3 | 23.41 | 0.09 | 0.38 | 23.44 | 0.37 | 1.58 | | 3 | 29.75 | 0.04 | 0.13 | 29.56 | 0.28 | 0.95 |
|
|