Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR

Table 3

Detection results of samples by conventional and real-time PCR.

Samples	Number	Methods
		Conventional PCR		SYBR Green I		TaqMan probe
		HP-PRRSV	PRRSV	HP-PRRSV	PRRSV	HP-PRRSV	PRRSV
Reference strains	4	2	2	2	2	2	2
Serum 1	15	6	4	6	5	6	6
Serum 2	39	0	5	0	6	0	8
Serum 3	477	2	0	19	0	34	7