Preferential Autoimmune Response in Prostate Cancer to Cyclin B1 in a Panel of Tumor-Associated Antigens
Figure 1
(a) Antibody titers to cyclin B1 in ELISA were expressed as optical density (OD) units. The mean + 3SD of normal human sera are shown in relationship to PCa and BPH. (b) Western blotting analysis shows antibody reactivity to cyclin B1 before and after absorption in nitrocellulose membrane strips blotted with purified recombinant cyclin B1 protein. Lanes 1–3 are sera from PCa patients and the corresponding OD values of these sera are indicated in panel (a). In general, OD values in ELISA were related to intensity of signals in Western blotting and the signals decreased obviously after sera absorption.
