Tolerogenic Splenic IDO⁺ Dendritic Cells from the Mice Treated with Induced-Treg Cells Suppress Collagen-Induced Arthritis

Figure 1

The adoptive transfer of iTregs suppressed the development of CIA. (a) The capacity of iTregs to inhibit CD4⁺T cell proliferation was assessed through CFSE staining. Different doses of iTregs were added to the proliferation assay, and the progressive dilution of CFSE in responder cells was used as a readout of proliferation. (b) CIA mice subjected to the adoptive transfer of iTregs or not were scored for clinical signs of arthritis in the limb joints through macroscopic examination three times a week and were sacrificed 49 days after the first immunization with CII. The arthritic scores in each group () were expressed as the means ± SEM. , compared with CIA mice using unpaired t-tests. (c) Hind paw specimens from recipient mice treated with or without iTregs were collected 3 weeks after the onset of CIA and were stained with Safranin O (showing cartilage erosion).

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