Tolerogenic Splenic IDO⁺ Dendritic Cells from the Mice Treated with Induced-Treg Cells Suppress Collagen-Induced Arthritis

Figure 3

The adoptive transfer of iTregs induced a significant inhibitory effect of splenic DCs on CIA. DC_CIA and DC_iTreg were pulsed with CII peptide and adoptively transferred into CIA mice at the same density (/animal). (a) Each group of mice () was scored for clinical signs of arthritis. The arthritic scores following the adoptive transfer of different DCs are shown. , compared with DC_iTreg-treated group and DC_CIA-treated group/untreated CIA group. (b) Hind paws were collected 3 weeks after the onset of CIA. The tissue was stained with H&E to visualize synovial joint inflammation. (c) The histopathological scores of inflammation were expressed as the means ± SEM of five independent experiments. compared with the indicated groups using unpaired t-tests. (d) Serum was collected from each group of mice 3 weeks after the onset of CIA. Serum levels of TNF-α, IL-17, IL-6, and TGF-β were measured using CBA and ELISA assays. The data are reported as the means ± SEM. , compared with the indicated groups using unpaired t-tests. The results of five replicated independent experiments were pooled. (e) Serum levels of total CII-specific immunoglobulin in each group of mice were determined using ELISA. The results of five replicated independent experiments were pooled.

(a)  

(b)

(c)

(d)

(e)