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Journal of Immunology Research
Volume 2014, Article ID 879897, 9 pages
http://dx.doi.org/10.1155/2014/879897
Research Article

Increased Levels of Granulocytic Myeloid-Derived Suppressor Cells in Peripheral Blood and Tumour Tissue of Pancreatic Cancer Patients

Yazan S. Khaled,1,2,3,4,5 Basil J. Ammori,1,2,3,4 and Eyad Elkord1,2,6

1Institutes of Cancer, Inflammation & Repair, University of Manchester, Manchester M20 4BX, UK
2Biomedical Research Centre, School of Environment & Life Sciences, University of Salford, The Crescent, Peel Building, Manchester M5 4WT, UK
3Department of Upper Gastrointestinal Surgery, Salford Royal NHS Foundation Trust, Manchester M6 8HD, UK
4Department of Hepatobiliary Surgery, North Manchester General Hospital, Manchester M8 5RB, UK
5Section of Translational Anaesthetic and Surgical Sciences, Leeds Institute of Molecular Medicine, Leeds LS9 7TF, UK
6College of Medicine & Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE

Received 26 September 2013; Revised 1 December 2013; Accepted 3 December 2013; Published 29 January 2014

Academic Editor: Steven Eric Finkelstein

Copyright © 2014 Yazan S. Khaled et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pancreatic cancer (PC) often presents late with poor survival. While role of immunosuppressive cells in preclinical studies provided help to develop immunotherapeutic agents, these cells remain under investigation in PC. The aim of this study was to characterise the different subsets of myeloid-derived suppressor cells (MDSCs) and evaluate their level and function in the circulation and tissue of PC patients. Significant increases in circulating and tumour-infiltrating granulocytic (Lin-HLA-DR-CD33+CD11b+CD15+), but not monocytic (Lin-HLA-DR-CD14+), MDSCs were detected in PC patients when compared with healthy donors and patients with chronic pancreatitis. The circulating MDSCs from PC patients expressed arginase 1, which represents their functional state. Blood levels of MDSCs showed no association with PC stage or preoperative levels of tumour markers. These findings provide a first characterisation of the phenotype of different subsets of peripheral and local MDSCs in PC patients and suggest that the frequency and contribution of these cells are predominantly granulocytic. This information demonstrates that MDSCs play a role in pancreatic cancer and future large validation studies may help in the development of new immunotherapeutic strategies to inhibit or eliminate MDSC function.