IL-27 Driven Upregulation of Surface HLA-E Expression on Monocytes Inhibits IFN- Release by Autologous NK Cells
Figure 1
Expression and function of IL27R and IL30R in human monocytes. (a) Expression of gp130 (white bar), WSX-1 (grey bar), and IL6R (light grey bar) was evaluated by flow cytometry in freshly isolated monocytes. Results are represented as MRFI. Histograms represent mean of six different experiments ± SD. One representative FACS analysis is reported in (b). Grey profiles show staining with irrelevant fluorochrome and isotype matched mAb. Black profiles show staining with specific mAb. (c) STAT1, STAT3, and ERK1/2 phosphorylation was investigated in monocytes cultured with medium alone (white bars), 100 ng/mL IL-27 (light grey bars), or 100 ng/mL IL-30 (grey bars). Results are represented as MRFI. Histograms represent mean of six different experiments ± SD. Asterisks indicate statistical significance. One representative FACS analysis is reported in (d). Grey profiles show staining with irrelevant fluorochrome and isotype matched mAb. Black profiles show staining with specific mAb.
