Rapamycin Regulates iTreg Function through CD39 and Runx1 Pathways

Figure 2

Rapamycin improved the expression of FoxP3 and developed the potent suppressive activity in vitro. (a) The proportion of CD4⁺CD25⁺FoxP3⁺ iTreg induced from naïve T cells. (b) CD25 and FoxP3 coexpression in iTreg was assessed by flow cytometry on day 7. (c) Relative CD25 MFI in iTreg cells on day 7 with or without rapamycin. (d) In this representative experiment, the cells were stained for anti-CD8 and the suppressive activity of various primed CD4⁺ cells subsets on CFSE-labeled CD8⁺ at various T suppressor to T effector ratios was shown. (e) The mean ± SEM percent suppression of iTreg at the various ratios. The values indicated the mean ± SEM of 3 separate experiments. ^∗𝑃<0.05.

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