Lactic Acid Bacteria Strains Exert Immunostimulatory Effect on H. pylori-Induced Dendritic Cells
Table 3
Effect of the examined bacteria and their mixtures on the expression of DCs markers (CD14, HLA-DR, CD80, CD83, CD86, and CD40).
Receptor type
Control
L. rhamnosus 900
L. paracasei 915
H. pylori
L. rhamnosus 900 + H. pylori
L. paracasei 915 + H. pylori
CD14
%
0.83 0.47–0.87
1.570.38–2.83
0.89 0.50–2.67
1.29 [0.85–2.18]
1.63 0.43–1.92
0.85 0.41–4.70
GFI
172 147–221
217 165–230
198 162–256
162 [149–179]
206 157–211
232 169–258
HLA-DR
%
95.42 94.80–99.21
96.21 94.90–98.11
98.21 95.60–98.50
98.70 [98.60–99.15]
96.85 95.09–97.30
96.75 94.30–99.11
GFI
335 294–360
778* 700–1806
765* 632–1423
1504* [1040–1811]
777* 717–1989
839* 713–1436
CD80
%
4.20 3.91–5.18
15.50*† 12.40–28.20
37.80*† [25.80–47.60]
3.71 [2.53–11.90]
18.5*† 13.10–29.10
36.65*†‡ 28.10–56.20
GFI
154 140–181
152 147–160
216*†‡ 166–224
154 [149–162]
149 148–170
177‡ 161–185
CD83
%
1.56 0.99–1.94
14.7* 8.95–18.88
27.27–45.00
6.27* [3.94–13.01]
13.35* 9.52–18.43
18.77–50.98
GFI
160 146–207
155 132–239
152 141–157
147 [140–159]
155 155–181
143 136–152
CD86
%
97.25 89.11–99.61
96.4595.18–99.10
96.30 95.66–99.43
99.00 [95.44–99.33]
97.45 95.71–99.55
97.12 94.96–99.35
GFI
554 373–608
2740* 1118–3390
2079.5* 899–3143
3388* [1514–3742]
3447* 3048–3667
2496* 2002–3226
CD40
%
35.51 17.33–40.41
30.2626.34–39.89
23.70 23.46–27.78
21.05 16.95–28.25
30.45 24.79–53.67
26.65 12.17–45.45
GFI
145 105.2–175
145 141–153
175 132–219
131 123–140
146 127–167
145 142–149
DCs: dendritic cells; %: the percentage of DCs expressing the analyzed receptor; GFI: geometric mean fluorescence intensity of the analyzed receptor in DCs population exhibiting its expression; values are expressed as the medians of six independent experiments and range of lower quartile-upper quartile Q1–Q3; statistically significant differences: *stimulators versus control (unstimulated DCs), †stimulators versus H. pylori, ‡stimulators versus L. rhamnosus 900, and stimulators versus L.rhamnosus 900 + H. pylori; P < 0.05.
