Infusion of Sulfosuccinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate-Conjugated MOG35–55-Coupled Spleen Cells Effectively Prevents and Reverses Experimental Autoimmune Encephalomyelitis in Mice
Effect of preadministration of MOG-coupled spleen cells on preventing EAE. (a) Female C57BL/6 mice were immunized with MOG35–55 peptide (200 μg) emulsified in CFA containing Mycobacterium tuberculosis H37Ra on day 0. The mice received i.p. injection of 200 ng pertussis toxin on day 0 and day 2. Mice were randomly divided into four groups (10 mice/group) and given intravenous injection of 1 × 107 different cells (UV-MOG-SPs, MOG-SPs, and SPs) or the same volume of PBS before EAE induction (designated as −d14 and −d7) and after EAE induction (days 0 and +7). All mice were monitored daily for over 2 months. The scores of treatment groups were compared with the scores of PBS treated group at each time point. ; . (b) Histology examination of spinal cords from the mice receiving the treatments above was performed on day 30 (4 mice/group). H&E (upper panel), LFB (middle panel), and NF staining (bottom panel) were performed to examine mononuclear infiltration, demyelination, and axonal injury in the lumbar spinal cords. Original magnification ×20. The arrows showed a stronger infiltration, increased demyelination, and more axonal loss in SPs and PBS treated groups compared with MOG-coupled spleen cells treatment. Similar data were obtained from two additional independent experiments.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.