Infusion of Sulfosuccinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate-Conjugated MOG_35–55-Coupled Spleen Cells Effectively Prevents and Reverses Experimental Autoimmune Encephalomyelitis in Mice

Figure 4

Adoptive transfer of CD4+ T cells leads to resistance to EAE induction. The splenic CD4+ T cells from three groups (treatment of SPs, UV-MOG-SPs, or MOG-SPs) were intravenously injected into naïve C57BL/6 mice (5 mice/group), respectively. After receiving CD4+ T cell transfer, all recipient mice underwent EAE induction on the same day. EAE development was monitored daily for 30 days. The scores of UV-MOG-SPs or MOG-SPs group were compared with the scores of SPs group at each time point. ; . Similar data were obtained from two additional independent experiments, and the donor cells were prepared from different mice in these three experiments.