Herpes Murine Model as a Biological Assay to Test Dialyzable Leukocyte Extracts Activity

Figure 4

In vitro evaluation of antiviral activity of Transferon. (a) HSV-1 was preincubated for 60 min with Transferon (20 μg/mL) or medium before evaluation of visible cytopathic effect (CPE) on Vero cells. Viruses with no preincubation (t₀) were used as control (ANOVA, Bonferroni’s Multiple Comparison Test; ns: nonsignificant). (b) Effect of Transferon (10 pg/mL–10 μg/mL) on HSV-1-induced cytopathology, evaluated by MTS assay. Acyclovir (ACV; 10 μg/mL) was included as a positive control. The graph represents data from 2 independent experiments (ANOVA, Bonferroni’s Multiple Comparison Test; ). (c) TCID₅₀ obtained from Vero cells preincubated for 24 h with Transferon (20 μg/mL) or medium prior to HSV-1 infection (Student’s t test). (d) Titration of samples obtained from Vero cells infected 24 h with HSV-1 and simultaneously treated with medium (open circles) or 20 μg/mL of Transferon (closed circles). ACV (10 μg/mL; triangles) was included as a positive control. Sum-of-squares F test showed no differences between the TCID₅₀ from medium- and Transferon-treated cells (). The graph represents data from 2 independent experiments.

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