Endothelin Receptors Expressed by Immune Cells Are Involved in Modulation of Inflammation and in Fibrosis: Relevance to the Pathogenesis of Systemic Sclerosis

Figure 2

ET_A and ET_B transcripts amplified by RT-PCR in fibroblasts, CD4+ T lymphocytes, activated CD4+ T cells, and neutrophils. ET_A corresponds to a molecular weight of 446 bp and ET_B to a molecular weight of 558 bp. (a) ET_A and ET_B transcripts amplified by RT-PCR in fibroblasts and neutrophils. Lane 1: molecular weight ladder; lane 2: negative control; lane 3: fibroblasts (ET_A); lane 4: neutrophils (ET_A), lane 5: negative control; lane 6: fibroblasts (ET_B); lane 7: neutrophils (ET_B). (b) ET_A transcripts amplified by RT-PCR in T lymphocytes, activated T cells, and fibroblasts. Lane 1: T lymphocytes; lane 2: activated T cells; lane 3: fibroblasts; lane 4: negative control, lane 5: molecular weight ladder. (c) ET_B transcripts amplified by RT-PCR in T lymphocytes, activated T lymphocytes, and fibroblasts. Lane 1: negative control; lane 2: T lymphocytes; lane 3: activated T cells; lane 4: fibroblasts; lane 5: molecular weight ladder.

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