Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity

Figure 3

U266 inhibit Collagen I (COLLI) and RUNX2 expression on normal human osteoblasts (hOBs). Human undifferentiated osteoblasts or hOBs differentiated for 2, 7, 14, or 21 days (Days of diff.), were cultured in the presence or absence of U266 or their conditioned medium, and then were analyzed for western blot analysis to detect the protein levels of COLLI (a) or RUNX2 (b). The histograms represent the mean optical density (OD) of COLLI or RUNX2 ratio normalized to the OD of -Actin. Data are presented as mean ± SE. The figure shows one representative of three independent experiments.

(a)

(b)