Induction of Apoptosis Coupled to Endoplasmic Reticulum Stress through Regulation of CHOP and JNK in Bone Marrow Mesenchymal Stem Cells from Patients with Systemic Lupus Erythematosus

Figure 4

The role of ERS in apoptosis of BM-MSCs from SLE patients. (a) BM-MSCs of SLE patients and normal controls were transfected with scramble siRNA (^#) and 40 nM CHOP siRNA respectively, for 48 h using the RNAifect transfection reagent. Western blot analysis was performed for CHOP and Bcl-2 respectively. (Bar represents mean ± SD, compared with the normal group, ^# compared with the SLE group). (b) BM-MSCs of SLE patients and normal controls were transfected with scramble siRNA (^#) and 50 nM PERK siRNA, respectively, for 48 h using the RNAifect transfection reagent. Western blot analysis was used for PERK and CHOP respectively. GAPDH was used as an internal control. (Bar represents mean ± SD, compared with the normal group, ^# compared with the SLE group). (c) Immunofluorescence analysis was performed for CHOP expression.

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