Induction of Apoptosis Coupled to Endoplasmic Reticulum Stress through Regulation of CHOP and JNK in Bone Marrow Mesenchymal Stem Cells from Patients with Systemic Lupus Erythematosus

Figure 5

The role of MAPK on ERS-induced apoptosis of BM-MSCs from SLE patients. (a) BM-MSCs of SLE patients and normal controls were treated with (+) or without (−) 4-PBA (100 μM) from the same time point for the indicated time. The levels of p-ERK1/2, total ERK1/2, p-JNK1/2, total JNK1/2, p-p38, and total p38 were detected by western blotting respectively. (b) Immunofluorescence analysis of p-JNK1/2 expression in the control group and SLE group treated with or without 4-PBA. (c) BM-MSCs of SLE patients and normal controls were transfected with scramble (^#) or 20 nM JNK1/2 siRNA for 48 h using the RNAifect transfection reagent. Western blot analysis was performed for p-JNK1/2 and BAX. (d) BM-MSCs of SLE patients and normal controls were transfected with scramble (^#) or 50 nM IRE-1siRNA for 48 h using the RNAifect transfection reagent. Western blot analysis was performed for p-JNK1/2 and p-IRE-1. (Bar represents mean ± SD, compared with the normal group, ^# compared with the SLE group).

(a)

(b)

(c)

(d)