IL-12 Inhibits Lipopolysaccharide Stimulated Osteoclastogenesis in Mice

Figure 2

Radiolucent areas in the mouse calvariae. (a) Microfocal computed tomography reconstructed images of calvariae. Images of calvariae excised from mice after daily supracalvarial administrations of PBS, LPS alone (100 g/day), LPS with IL-12 (1.5 g/day), or IL-12 alone for 5 days. The arrows show the resorption lacunae. (b) Evaluation of the radiolucent areas on the calvariae. The radiolucent areas in the dotted boxed area shown in the right side of the graph were calculated. The red areas indicate the radiolucent areas. The relative values of the radiolucent areas in the groups were normalized by the corresponding values in the PBS group. (c) Serum levels of TRACP 5b. Circulating TRACP 5b levels were determined by ELISA. Results are expressed as means ± SD ( = 9; < 0.01). Differences were detected using Scheffe’s tests.

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