CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a

Figure 2

Cyp4f18 knockout neutrophils show increased C5a-dependent chemotaxis compared to wild-type. Chemotaxis of bone marrow neutrophils from wild-type (WT) and Cyp4f18 knockout (KO) mice was measured using C5a as a chemoattractant. Prior to chemotaxis, the neutrophils were incubated with or without the FLAP inhibitor MK 886 (0.5 μM), a cPLA2α inhibitor (CID 9833099, 1 μM), or a BLT1 antagonist (LY223982, 10 μM). (a) Chemotactic index represents the number of cells migrated in response to C5a divided by background (error bars represent SEM, , , ). (b) Schematic plot for the relative difference in chemotactic index in Cyp4f18 knockout neutrophils compared to wild-type for each experimental condition.

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