CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a

Figure 3

Gene expression in wild-type and Cyp4f18 knockout neutrophils. Flow cytometry analysis of bone marrow cells from wild-type (WT) and Cyp4f18 knockout (KO) mice using antibodies to Ly6G (neutrophil marker) and C5aR; representative double plots from individual mice are shown (a). Reverse transcription and real time PCR analysis of C5aR mRNA expression (b) and CYP4 mRNA expression (c), in isolated bone marrow neutrophils. ΔCt values were determined for each transcript using GAPDH as endogenous control, and values were plotted for WT and KO samples (). Relative quantitation () determined that there was no significant change in expression of any of the transcripts tested in knockout samples compared to wild-type, except for the loss of CYP4F18.

(a)

(b)

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