A Novel PET Imaging Using ⁶⁴Cu-Labeled Monoclonal Antibody against Mesothelin Commonly Expressed on Cancer Cells

Figure 5

In vitro and in vivo stability of ⁶⁴Cu-DOTA-11-25 mAb and ⁶⁴DOTA-anti-KLH mAb. (a) In vitro stability of ⁶⁴Cu-DOTA-11-25 mAb (black bars) and ⁶⁴DOTA-anti-KLH mAb (white bars) after 24 hours and 48 hours of incubation at 37°C. Radioactivity derived from antibody-bound ⁶⁴Cu stays at the origin and unbound ⁶⁴Cu goes to the top of the chromatogram. The percentage of radioactivity that remained in the origin of TLC was shown as the stability. (b) Representative TLC-ARG of plasma and supernatant of homogenate from liver and kidney 24 hours after injection of ⁶⁴Cu-DOTA-11-25 mAb. Aliquot of blood, liver, and kidney was taken from mice 24 hours after injection and analyzed by TLC-ARG as described in Section 2. (c) In vivo stability of ⁶⁴Cu-DOTA-11-25 mAb (black bars) and ⁶⁴Cu-DOTA-anti-KLH mAb (white bars) 24 and 48 hours after injection.

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